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M94A0630.TXT
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1994-10-21
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Document 0630
DOCN M94A0630
TI Activation of the NF-kappa-B p105 promoter by HTLV-I Tax.
DT 9412
AU Lambert P; Ludford M; Deacon N; Doherty R; Macfarlane Burnet Centre for
Medical Research, Fairfield,; Victoria.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:69 (abstract no. FB5).
Unique Identifier : AIDSLINE ASHM5/94349025
AB The transactivator protein of HTLV-I, Tax, activates transcription not
only from the HTLV-I LTR, but also from several other viral and cellular
promoters. One of its targets is the LTR of HIV; HIV replication is
enhanced in HTLV-I-infected cell lines, and there is increasing evidence
that patients infected with both viruses show a more rapid progression
to the diseased states associated with HIV infection. Activation of the
HIV LTR by Tax is mediated by proteins of the NF-kappa B(NF-kappa
B)/c-Rel system; there are two NF-kappa B target sites in the HIV LTR.
Tax induces nuclear localization of cytoplasmic NF-kappa B/c-Rel,
allowing it to bind to its target sites and activate transcription.
There is also the possibility that Tax can increase the de novo
synthesis of the NF-kappa B/c-Rel proteins. This seems especially likely
given that at least some of them regulate their own synthesis. The gene
encoding the precursor of NF-kappa B p50, p105, is one such gene.
Reports vary on whether or not Tax can stimulate its promoter, and such
activity may be cell type-dependent. Our preliminary results with Jurkat
T cells indicated that a cotransfected Tax-expressing construct could
activate a p105 promoter-CAT reporter plasmid, and we undertook further
experiments to determine the mechanism for this.
DE Cell Line Gene Amplification/*GENETICS Gene Expression Regulation,
Viral/PHYSIOLOGY Gene Products, tax/*GENETICS Human HIV-1/GENETICS
HTLV-I/*GENETICS NF-kappa B/*GENETICS Promoter Regions
(Genetics)/*GENETICS Protein Precursors/*GENETICS Virus
Replication/*GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).